First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype. To distinguish between these possi-bilities, the Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double-mutant mice

2001-03-01 · Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA.

Required also for telomere recombination to repair telomeric ends in the absence of telomerase. ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background . XRCC4, is a protein of unknown function. The Ku70 protein is an additional component of DNA-PK; Ku70 forms a het-erodimer with Ku80 to generate the DNA end-binding com-ponent of the enzyme. To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression. We find Ku70 is one component of a protein complex, Ku70 and Ku80, that functions as a heterodimer to bind DNA double-strand breaks and activates DNA-dependent protein kinase. Our previous study with Ku70−/− and Ku80−/− mice, and cell lines has shown that Ku70- and Ku80-deficiency compromises the ability of cells to repair DNA double-strand breaks, increases radiosensitivity of cells, and Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection.

2011-10-01 · Ku, the heterodimer of Ku70 and Ku80, plays an essential role in the DNA double-strand break (DSB) repair pathway, i.e., non-homologous end-joining (NHEJ). Two isoforms of Ku80 encoded by the same genes, namely, Ku80 and KARP-1 are expressed and function in primate cells, but not in rodent cells.

Sweetwater engineers are excited about this function, which makes KH 80 ideal for surround sound monitoring configurations. The KH 80 DSP nearfield studio

2013-07-01 There was no correlation between the transcript levels of Ku80 and LUC luminescence derived from T‐DNA stable transformation in KD‐OsKu80 rice calli (Figs 1b, 2d,e). It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994). 2016-07-08 DNA-PKcs functions both within and outside of the damage response to effectively design therapeutic strategies.

Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Ku70 and Ku80 form the “Ku” DNA end-binding complex which functions as the DSB recognition com-ponent of C-NHEJ, whereas the XRCC4/ Lig4 complex is specific for C-NHEJ ligation. (2002) Korabiowska et al. Modern Pathology. Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks. Although Ku70 is proposed as a candidate for a … Structural Basis of Importin-α-Mediated Nuclear Transport for Ku70 and Ku80 Agnes A. S. Takeda1, Andrea C. de Barros1, Chiung-Wen Chang2, Boštjan Kobe2 and Marcos R. M. Fontes1⁎ 1Departamento de Física e Biofísica, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP 18618-970, Brazil 2School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience 2003-03-01 of the Ku70/80 heterodimer in vitro. We further deﬁned the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction. 2.

When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Ku80 is a protein that, in humans, is encoded by the XRCC5 gene. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair. We developed a novel assay to measure DNA binding and release kinetics using differences in Förster resonance energy transfer (FRET) of the ECFP-Ku70/EYFP-Ku80 heterodimer in soluble and DNA end bound states. Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks.
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It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system .

The Ku antigen is a heterodimer of Ku70 and Ku80.
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The Ku heterodimer, comprised of Ku70 and Ku80 subunits, is a conserved complex involved in nonhomologous end-joining (NHEJ). However, it also functions in maintenance of telomeres, chromosome

Two isoforms of Ku80 encoded by the same genes, namely, Ku80 and KARP-1 are expressed and function in primate cells, but not in rodent cells. Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation.

Cell Reports Article PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF Satish K. Tadi,1 Carine Tellier-Lebe`gue,2 Cle´ment Nemoz,2 Pascal Drevet,2 Ste´phane Audebert,1 Sunetra Roy,3,4

Keywords Autapomorphy.Centromere. Eyprepocnemisplorans.Geneknockdown. Immunofluorescence.Kinetochore.Ku70.Ku80. Microtubules.Orthoptera.RNAi.Spindleassembly checkpoint Abbreviations Ku70 and Ku80. Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986 ). Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( … We conclude that Ku70 and Ku80 may have functions in V (D)J recombination and DNA repair that are independent of DNA-PKcs. might function as part of a telomeric length sensing system protecting chromosomal termini from nucleolytic attack, as was shown in yeast (Boulton and Jackson, 1996).

Ku70 is the 70 kDa subunit of the lupus Ku autoantigen.